内皮细胞生长因子对人胆囊上皮细胞培养的影响

Effects of endothelial cell growth factor on human gallbladder epithelial cells culture

目的 :探索内皮细胞生长因子 (EGF)对人胆囊上皮(HGBEC)细胞体外培养的影响 .方法 :培养基中添加EGF进行细胞培养 ,与培养基中未添加EGF组进行比较 .结果 :添加EGF组胆囊上皮细胞数目 (平均 4 .6× 1 0 7个 /孔 )较未添加组 (2 .6× 1 0 7个 /孔 )增多 (P <0 .0 5 ) ,存活时间明显延长 (从 8.3d增加到 1 8.6d) (P <0 .0 5 ) ,第 6～ 7日细胞数达最高值 ,可维持 1 0～ 1 2d ,细胞最长可存活 2 5d.结论

AIM: To explore the effects of endothelial cell growth factor (EGF) on human gallbladder epithelial cells (HGBEC) culture in vitro . METHODS: HGBEC were cultured in DMEM medium with EGF and compared with those in control group without EGF. RESULTS: The number of HGBEC in test group was more than that in control group (4.6×10 7/aperture vs 2.6×10 7/aperture, P <0.05), so was the duration of HGBEC in test group. From the sixth to seventh day, the number of HGBEC was the highest and the longest duration was 25 d. CONCLUSION: EGF added to DMEM medium can prolong the duration of HGBEC and increase the number of HGBEC.