抑癌基因PTEN在子宫内膜癌组织中的表达

Expression of tumor suppressor gene PTEN in endometrial carcinoma

目的 :了解抑癌基因PTEN在子宫内膜癌中的表达及与子宫内膜癌临床病理因素的相关性 ,探讨其与子宫内膜癌发生发展的关系 .方法 :运用免疫组织化学SABC法测定PTEN蛋白在 77(子宫内膜样腺癌 72 ,子宫浆液性癌 5 )例子宫内膜癌组织和 2 5例正常子宫内膜中的表达 ,并比较PTEN表达变化与组织学类型、手术临床分期、病理分级、子宫肌层浸润程度、淋巴结转移及患者年龄等临床病理因素之间的相关性 .结果 :子宫内膜癌组织中PTEN表达缺失率为 6 0 % ,与正常子宫内膜相比 (0 % )存在显著性差异 (P =0 .0 0 0 0 ) .子宫内膜样腺癌中PTEN的失表达率为 6 4 % ,而子宫浆液性癌为0 % ,二者差异显著 (P =0 .0 0 4 8) .PTEN的失表达率随内膜癌恶性程度的增加而增加 (P =0 .0 0 34) ,但与手术临床分期、肌层浸润程度、淋巴结转移及患者年龄等因素无关 (P >0 .0 5 ) .结论 :抑癌基因PTEN是在子宫内膜癌发生过程中占重要地位的突变基因 ,PTEN基因的失表达与子宫内膜癌 。

AIM: To investigate the effects of tumor suppressor gene PTEN in the carcinogenesis and development of the endometrial carcinoma and to identify the correlations between the expression of PTEN and the clinicopathologic factors of endometrial carcinoma. METHODS: Seventy seven endometrial carcinoma samples were immunohitochemically analyzed, 72 of which were adenoendometrial carcinoma and 25 normal endometrium samples for PTEN expression. Results were compared with the clinicopathologic factors, such as type, stage, grade, myometrial invasion, lymph invasion and age. RESULTS: Loss of PTEN protein expression in endometrial carcinoma (60%) was much more frequent than those of normal endometrium tissues (0%, P = 0.0000) and the expression level of PTEN varied between the two different histological types (64% vs 0%, P = 0.0048) and the pathological grade. Loss of PTEN protein expression increased with the malignancy grade of endometrial carcinoma ( P =0.0034) but was not associated with clinical stages, myometrial invasion, lymph invasion and ages. CONCLUSION: Tumor suppressor gene PTEN plays an important role in the carcimogenesis and development of the endometrial carcinoma. The loss of PTEN expression is closely related to the carcinogenesis and development of the endometrial carcinoma, especially the adenoendometrial tumorigenesis.