乙二醛酶-Ⅰ单核苷酸多态性对糖尿病周围大血管并发症的影响

The single nucleotide polymorphism of glyoxalase-Ⅰ gene C329A associated with diabetic peripheral macrovascular complications

目的 研究乙二醛酶 Ⅰ基因单核苷酸多态性与糖尿病周围大血管病并发症的相关性。方法 收集糖尿病有、无周围大血管并发症DNA样本各 3 6例及 46例 ,正常对照 49例。采用聚合酶链反应结合测序的方法。结果 检出C3 2 9A和A3 69T两个多态位点。糖尿病有周围大血管病变组C3 2 9A位点AA型基因型频率 72 .2 %明显高于糖尿病无周围大血管并发症组的 5 0 .0 % (P <0 .0 5 )和正常人组的 49.0 % (P <0 .0 5 ) ,通过 χ2 检验 ,此位点为AA型患周围血管病变的危险性增加(OR =2 .6,95 %可信限为 1.0 2 5～ 6.5 93 ,P <0 .0 5 )。糖尿病有、无周围大血管病变组及正常人组的A3 69T位点AA基因型频率分别为 5 5 .6%、60 .9%及 61.2 % ,三组间无显著差异。结论 乙二醛酶 IC3 2 9A位点AA基因型与糖尿病周围大血管并发症有相关性。

Objectives To investigate the association of single nucleotide polymorphisms (SNPs) of glyoxalase-Ⅰ gene with diabetic peripheral macrovascular complications.Methods Total 36 diabetic patients with,46 without diabetic peripheral macrovascular complications and 49 normal controls were recruited.SNPs of glyoxalase-Ⅰ gene were genotyped by polymerase chain reaction (PCR) and subsequent direct sequencing.Results Two SNPs were found in the gene,A C/A at nucleotide (nt) position 329 and another A/T at nt position 369.The frequency of genotype AA of glyoxalase-Ⅰ gene at 329 were significantly higher in patients with peripheral macrovascular complications (72.2%) than that in patients without the complications (50.0%,P<0.05) and that in normal controls (49%,P<0.05),Chi-square test showed that 329AA in type 2 diabetic patients associated with a 2.6-fold increase in risk of diabetic peripheral macrovascular complications (95%CI: 1.025 to 6.593,P<0.005).No significant difference of 369AA of glyoxalase-Ⅰ gene were seen among diabetic patients with (55.6%),without (60.9%) peripheral macrovascular complications and controls (61.2%)(P>0.05).Conclusion Genotype 329AA of glyoxalase-Ⅰ gene were associated with diabetes peripheral macrovascular complications.