血管内皮细胞生长因子对移植后骨髓造血微环境的影响及意义

The Effects and Significance of Vascular Endothelial Growth Factor on Hematopoietic Inductive Microenvironment in Post-BMT Mice

目的 观察血管内皮细胞生长因子基因治疗对骨髓移植后造血微环境的调节作用。方法 重组腺病毒注射给小鼠 ,随后进行同基因骨髓移植 ;在不同时期检测骨髓微血管灌注面积和造血细胞容量以及外周血WBC、PLT计数。结果 移植后 2 0天时 ,VEGF组骨髓微血管灌注面积已恢复正常 (P >0 .0 5 ) ;移植后 3 0天时VEGF组骨髓细胞容积明显大于EGFP组和常规组 (P <0 .0 5 )。各时间点VEGF处理组外周血WBC、PLT计数均显著高于常规BMT组和EGFP组 (P <0 .0 5 )。结论 血管内皮细胞生长因子基因治疗促进了骨髓移植后造血微环境的恢复 ,并提高外周血WBC、PLT计数水平。

Objective To observe the regulation of hematopoietic inductive microenvironment by VEGF gene treatment in post-bone marrow transplantation mice.Methods Adenovirus was injected into mice accepted syngeneic bone marrow transplantation(BMT).At different phases post BMT,the values of microvasculature surface area and cellularity of bone marrow were tested as well as the counts of white blood cells(WBC) or platelets(PLT) were measured.Results The percentage of microvasculature surface area in group VEGF returned to normal level at +20 days post-BMT(P>0.05).At +30 days post-BMT,the percent cellularity of BM in group VEGF was higher than those in group of AdEGFP or BMT alone(P<0.05).At each time,a rapid recovery of WBC or PLT was obtained in the group VEGF compared to groups treated with Ad-EGFP or BMT alone.Conclusion VEGF gene treatment facilitated the reconstruction of hematopoietic inductive microenvironment post BMT,and increased the numbers of WBC and PLT in peripheral blood.