聚合酶链反应杂交梳法鉴定结核杆菌

The Clinical Value of Polymerase Chain Reaction in Detection of M. tuberculosis

目的:探讨一种快速、简便、特异性较强的结核菌鉴定方法。方法:采用聚合酶链反应(PCR)结合反向探针膜杂交对痰液标本中的结核杆菌的特异性DNA片段进行检测。结果:32例阳性标本经过聚合酶链杂交结果与常规鉴定结果相符。结论:聚合酶链反应杂交梳法鉴定结核杆菌简便、快速、特异性强,有较高的临床应用推广价值。

Objective To research a quick,simple and highly specific method to detect the M. tuberculosis(TB). Methods Using polymerase chain reaction combining with inverse probe membrane hybridization to detect the specific DNA segments of TB in sputum. Results The detective results of 32 positive samples with PCR hybridization combing is a quick,simple and highly specific method in the detection of TB,and may has some values in clinical diagnosis.