摘要
The effect of trace metals of Zinc （Zn）, Selenium （Se） and Manganese （Mn） on thegrowth of a marine microalga was determined by culturing Nannochloropsis oculata in f/2 medium enrichedwith different concentrations of the metal compounds. The results showed that concentration and type ofthe metal compound affected the growth of Nannochloropsis. ZnCl<sub>2</sub> and Na<sub>2</sub>SeO<sub>3</sub>, promoted the growth ofNannochloropsis at relatively low concentrations of 15. 3 μmol/L and 57. 8 μmol/L respectively, but inhib-it its growth at higher concentration of 153 μmol/L and about 300 μmol/L. No significant growth promotionwas observed for MnCl<sub>2</sub> and MnSO<sub>4</sub> although MnCl<sub>2</sub> was found to have higher toxic effect than MnSO<sub>4</sub>.
The effect of trace metals of Zinc (Zn), Selenium (Se) and Manganese (Mn) on the growth of a marine microalga was determined by culturing Nannochloropsis oculata in f/2 medium enriched with different concentrations of the metal compounds. The results showed that concentration and type of the metal compound affected the growth of Nannochloropsis . ZnCl2 and Na, SeO3 promoted the growth of Nannochloropsis at relatively low concentrations of 15 .3 μmol/L and 57 .8 μmol/L respectively, but inhibit its growth at higher concentration of 153 μmol/L and about 300 μmol/L. No significant growth promotion was observed for MnCl2 and MnSO4 although MnCl2 was found to have higher toxic effect than MnSO4 . Nannochloropsis growth was more sensitively inhibited by ZnSO4 , ZnAc2 , MnCl2 and NaHSeO3 than by other types of compounds. The promotion effect not being observed in the above compounds, even at the lower concentration levels of the metal elements, indicated that different metal salts have different effect on Nannochloropsis growth. The inhibition of algal growth was commonly increased with high concentration of metal elements. Combination of two or three metais resulted in stronger inhibition of algal growth. Zn concentration in the algal cell could reach 492 mg/g when the concentration of ZnCl2 in the culture was 459 μmol/L, which was about 40 times that of control samples with concentration of ZnCl2 at 12 mg/g. Treatment of the algal cuture with 220.2 μmol/L ZnCl2 caused the decrease of amino acid in Nannochloropsis cells.
基金
This work was supported by Key Science Project of Fujian Province and by the Ministry of Education Foundation for University Key Teachers
