高血压脑出血半暗带早期病理变化及脑保护的实验研究

The research about early pathological change in penumbra area and brain protection after hypertensive intracerebral hemorrhage

目的研究高血压脑出血后血肿周半暗带的病理变化及早期保护措施。方法通过立体定向手术脑尾状核胶原酶加肝素联合注射诱导大鼠出血模型,采用30只卒中倾向的自发性高血压大鼠(SHRSP),体重200～230 g,随机分为2组,观察组和携氧液组。治疗组于血肿形成早期给予携氧液治疗,观察血肿周组织形态及超微结构。结果出血后6小时,观察组血肿周边神经细胞就普遍变性,大量脱髓鞘变化,并有凋亡小体形成。携氧液组患侧组织形态学及细胞超微结构改变明显减轻。结论高血压脑出血后血肿周边半暗带早期就产生显著的病理改变,携氧液对高血压的血肿周脑组织有良好的保护作用。

Objective To study the early pathological changes in penumbra area and brain protection after hypertensive intracerebra hemorrhage. Methods Collagenase and heparin were injected into the caudate nucleus of the stroke-prone spontaneously hypertensive (SHRSP) rats by sterotactic operation to induce a hemorrhage model. Early morphology of the brain surrounding hematomas (Group A) was observed, and oxygen-carrying liquid was given in treatment group (Group B). Results6 hours after hemorrhage, it was observed that the cerebral injury in group A was more serious than that in group B, including widespread neurocell degeneration, demyelination, meanwhile the changes of morphology in group B were less than group A. Conclusion The early pathological changes caused by hypertensive intracerebral hemorrhage appear in penumbra area around the hematoma. Oxygen-carrying liquid has good effect on brain tissue protection.