摘要
Objective To investigate the correlation between transcript CD 44S , CD 44V and gastric carcinoma invasion metastasis, and to know what role the CD 44 gene's abnormal activity plays in the genesis and development of gastric cancer. Methods RT-PCR was used to detect mRNA of CD 44V and CD 44S in 10 gastric normal mucosa,8 gastric precancerous mucosa,10 tissue adjacent to cancer,40 gastric carcinoma (including metastatic focus) with different pathological features.Results All gastric carcinomas and non-cancerous tissues expressed CD 44S ,but CD 44V expression rate were 0%, 12.5%,20% and 72.5% ,respectively, in normal gastric mucosa, precancerous tissue,tissue adjacent to cancer,carcinoma. Cancer invasion limited to submucosal layer showed lower CD 44V expression rate than invasion extended beyond the muscle layer (P<0.05). Samples with lymph node metastasis showed higher CD 44V expression rate than samples without lymph node metastasis,meanwhile, there was significant correlation between metastasis limited to N1 and beyond N1 (P<0.05). There was no relationship between CD 44V expression and Lauren's type, TNM stage as well (P>0.05). Conclusion There is a close relation between CD 44V and genesis and development of gastric cancer. CD 44 gene variant splicing is an early genetic affair in gastric cancer.It could be a diagnostic tool to screen high-risk people for gastric carcinoma. CD 44V can be regard as a parameter in evaluating the potential of cancer cell invasion and metastasis ,as well as prognosis.
Objective To investigate the correlation between transcript CD 44S , CD 44V and gastric carcinoma invasion metastasis, and to know what role the CD 44 gene's abnormal activity plays in the genesis and development of gastric cancer. Methods RT-PCR was used to detect mRNA of CD 44V and CD 44S in 10 gastric normal mucosa,8 gastric precancerous mucosa,10 tissue adjacent to cancer,40 gastric carcinoma (including metastatic focus) with different pathological features.Results All gastric carcinomas and non-cancerous tissues expressed CD 44S ,but CD 44V expression rate were 0%, 12.5%,20% and 72.5% ,respectively, in normal gastric mucosa, precancerous tissue,tissue adjacent to cancer,carcinoma. Cancer invasion limited to submucosal layer showed lower CD 44V expression rate than invasion extended beyond the muscle layer (P<0.05). Samples with lymph node metastasis showed higher CD 44V expression rate than samples without lymph node metastasis,meanwhile, there was significant correlation between metastasis limited to N1 and beyond N1 (P<0.05). There was no relationship between CD 44V expression and Lauren's type, TNM stage as well (P>0.05). Conclusion There is a close relation between CD 44V and genesis and development of gastric cancer. CD 44 gene variant splicing is an early genetic affair in gastric cancer.It could be a diagnostic tool to screen high-risk people for gastric carcinoma. CD 44V can be regard as a parameter in evaluating the potential of cancer cell invasion and metastasis ,as well as prognosis.
