糖尿病患者尿液多项特种蛋白、尿酶及细胞因子的临床意义。

The clinical value of urinary special proteins NAG and cytokines in patients with diabetes

目的 探讨糖尿病患者尿多项特种蛋白、尿酶、细胞因子在糖尿病早期肾脏损伤的诊断价值。方法 应用免疫比浊法、终点法及ELISA法分别检测尿mAIB、TRF、Fn、IgG、RBP、β_2-MG、NAG、IL-6、IL-8水平。结果 糖尿病尿蛋白定性阴性时尿各项特种蛋白、NAG皆高于正常对照组;尿蛋白阳性组以上各项指标均明显高于尿蛋白阴性组,尿蛋白阳性组IL—6、IL—8明显增高。结论 联合检测特种蛋白、尿酶可作为早期诊断糖尿病肾脏损伤的的灵敏招标,而IL—6、IL—8等细胞因子检测可反映肾损伤的严重程度。

Objective To study the value of urinary special proteins, N-acetyl-β-D-glucosaminidase(NAG)and cytokines in the diagnosis of earlier renal diseases in patients with diabetes. Methods The levels of urinary mALB, TRF, Fn, IgG, RBP, β_2-MG, NAG, IL-6 and IL-8 were determined with immunoturbidimetry, endpoint or ELISA methods. Results The levels of urinary special proteins and NAG in diabetic patients with negative urinary albumin were higher than those in healthy controls. And those in positive urinary albumin group were higher than in negative urinary albumin group. IL-6 and IL-8 in urine had risen significantly in the group with positive urinary albumin. Conclusion Together with urinary special proteins, NAG can be a sensitive item in the diagnosis of earlier renal disease in patients with diabetes. The determintion of IL-6 and IL-8 can indicate the degree of renal injuries, and it may have some value in pathogenesis of diabetic nephropathy and state of these diseases.