VCAM-1和ECP在支气管哮喘发病中作用机制的研究

The effect of serum VCAM-1 and ECP in bronchial asthmatic patients

目的 探讨血清可溶性血管内皮细胞粘附分子 1(sVCAM 1)和嗜酸性粒细胞阳离子蛋白 (ECP)在支气管哮喘发病中的作用机制。方法 对年龄在 15～ 4 5岁的 2 3例支气管哮喘患者 (9例为发作期 ,14例为缓解期 )和 2 0例健康人采用酶联免疫双抗体夹心实验 (ELISA法 )测定了sVCAM 1,应用荧光免疫法测定了血清ECP、血清总IgE(tIgE)和特异性IgE(sIgE)。结果 哮喘组的血清sVCAM 1(几何均数 783 2 0± 1 5 7ng ml)显著高于对照组 (几何均数 5 4 1 10± 1 5 4ng ml) ,P值 =0 0 0 9;两组血清ECP含量未见明显差异 ,但哮喘急性发作期的血清ECP(16 2 2± 2 90 μg L)水平明显高于缓解期病人的ECP(3 0 5± 1 80 μg L)P <0 0 0 1;血清总IgE几何均数比较 ,哮喘组 (474 5 0± 2 38kU L)显著高于对照组 (2 4 34kU L± 2 2 5kU L)P <0 .0 0 1;血清特异性IgE分析提示蒿草花粉和户尘螨的sIgE不仅阳性检出率高 ,而且其血清sIgE的含量亦较高。结论 支气管哮喘患者的血清sVCAM 1较正常人高 ,这可能与嗜酸性粒细胞的移行有关 ;血清ECP的含量变化与哮喘患者的疾病状态有关 ;

Objective To evaluate the role of vascular cell adhesion molecule 1(VCAM 1),eosinophil cationic protein(ECP),total IgE(tIgE) and specific IgE(sIgE) in bronchial asthma. Methods The serum soluble VCAM 1 in 23 bronchial asthma patients (9 cases during bronchial asthma attacks, 14 cases in stable conditions) and in 20 normal persons were detected by ELISA. Serum ECP,tIgE and sIgE were assayed with Pharmacia CAP system. Results The geometric mean of serum sVCAM 1 in patients (783 20±1 57ng/ml) were significant higher than that in normal controls (541 10±1 54ng/ml), P <0.01. The geometric mean of ECP in sera obtained during bronchial asthma attacks(16 22±2 90μg/L) were higher than that in sera obtained in stable condition(3 05±1 80μg/L), P <0 001. The positive rate of artemisia pollen IgE were (83.9%, 19/23). The positive sIgE of Ddermatophagoides pteronyssinus were 12 cases in 23 patients. The values of sIgE of artemisia pollen and Dermatophagoides pteronyssinus were higher than that of other allergens. The geometric mean of serum total IgE in patients (474 50±2 38kU/L) was higher than that in normal persons (24 34kU/L±2 25kU/L) P < 0.001; Conclusion The elevation of serum sVCAM 1 in patiens with bronchial asthma were observed. The content of serum ECP during bronchial asthma attacks is elevated. The seasonal allergen caused asthma in north China are mainly artemisia pollen,whereas perennial allergen are mainly house dust mite.