益心康胶囊对在体家兔心肌缺血/再灌注损伤保护作用的实验研究

Experimental Study of Protective Effects of Yixinkang Capsule on Myocardial Ischemia/Reperfusion Injury in Rabbits

目的:观察中药复方益心康胶囊的抗心肌缺血/再灌注损伤作用。方法:利用家兔心肌缺血30 min/再灌注60 min 损伤模型,观察益心康胶囊对损伤后超氧化物歧化酶(SOD)活性、丙二醛(MDA)含量、血清酶活性、梗塞范围、心电图改变及心肌代谢产物乳酸水平的影响。结果:益心康胶囊预防性给药能明显升高再灌注后的血 SOD 活性,减少 MDA 的形成,改善代谢,降低乳酸含量,降低血清肌酸磷酸激酶(CPK)及乳酸脱氢酶(LDH)水平,缩小梗塞范围,并显著改善缺血期及再灌注期的心电图变化。结论:益心康胶囊具有明显的抗缺血/再灌注损伤作用。

Objective:To observe the effects of Yixinkang(YXK) Capsule on myocardial ischemia/reperfusion injury.Methods:Using the mod- el of myocardial ischemia 30 min/reperfusion 60 min of coronary artery in rabbit in vivo,the protective effects of YXK(50,100mg/kg) were observed on superoxide dismutase (SOD),creatine phosphokinase (CPK),lactate dehydrogenase (LDH),malondialdehyde (MDA),and lactic acid (LA)in plasma,as well as the myocardium infarction size (MIS)and ECG.Results:The levels of CPK and LDH were markedly lowered,the MDA formation alleviated,the metabolism improved,the LA reduced,the MIS were significantly abated, and the activity of SOD were higher in YXK group than that of the control group.Conclusion:Experimental results revealed that YXK had obviously protective effects against myocardial ischemia/reperfusion injury in rabbits.