摘要
目的:观察大黄对急性肝内胆汁淤积大鼠的治疗效应,从蛋白、分子水平探讨大黄治疗肝内胆汁淤积的机制。方法:采用异硫氰酸萘酯(ANIT)建立大鼠急性肝内胆汁淤积模型,观察大黄组、ANIT 组不同时段血清胆红素(SB)值、丙氨酸转氨酶(ALT)活性、脂质过氧化物(LPO)含量、肝组织中肿瘤坏死因子-α(TNF-α)水平及2h 内胆汁流量变化。结果:大黄组 SB、ALI、LPO、TNF-α在灌服 ANIT 后24、48h 较 ANIT组均有明显降低,胆汁流量明显增加,差异有显著性(P<0.05)。结论:大黄能降低 SB,促进胆汁分泌,抗LPO,降低或直接拮抗 TNF-α致肝损害作用。
Objective:To observe the therapeutic effect of rhubarb in treating acute intraheaptic cholestasis(AIHC)for exploring its mechanism from protein and molecular levels.Methods.The rat AIHC model was established by α-naphthyl isothiocyanate(ANIT).Changes of serum bilirubin(SB),alanine transaminase(ALT) activity,lipid peroxidase(LPO)content,tumor necrosis factor-α(TNF-α)as well as the bile flow/2 hrs in different time periods in the rhubarb group and the control group were observed.Results.Levels of SB,ALT,LPO and TNF-α were markedly lower and the bile flow/2 hrs was higher in the rhubarb group than those in the control group with significant difference(P<0.05).Conclusion:Rhubarb could lower SB,promote bile secretion,resist LPO,reduce or directly antagonize the damage of liver by TNF-α.
出处
《中国中西医结合杂志》
CAS
CSCD
北大核心
2001年第S1期75-77,共3页
Chinese Journal of Integrated Traditional and Western Medicine
关键词
大黄
肝内
胆汁淤积
rhubarb
intrahepatic
cholestasis
