华蟾素对喉癌细胞生长及癌基因表达的影响

Effects of Cinobufacini on Human Laryngeal Carcinoma Cell Growth and Expression of Oncogene

目的:探讨中药华蟾素(cinobufacini,CIN)对体外培养人喉癌 Hep-2细胞生长作用及癌基因表达的影响。方法:应用光镜、电镜、MTT 检测技术及 SP 免疫组化技术,对药物作用后 Hep-2细胞的数量、形态、癌基因 p53及 c-myc 蛋白的表达进行研究。结果:华蟾素对 Hep-2细胞作用在低浓度短时间时促进细胞分裂,增殖和诱导细胞分化;高浓度长时间则抑制细胞生长,促进细胞凋亡。华蟾素对 Hep-2细胞癌基因影响表现为 c-myc 蛋白表达降低,p53蛋白表达变化不明显。结论:华蟾素对喉癌细胞的生长及癌基因有抑制作用,可作为临床喉癌综合治疗的用药之一。

To investigate the effects of cinobufacini(CIN)on oncogenes in human laryngeal carcinoma cell line Hep-2.Methods: The effect of CIN on the proliferation of Hep-2 calls was assessed by using the optical and transmission electron microscopy(TEM)to observe the morphologic change of cells.The cytotoxicity of CIN to the cell line was measured by methyl thiazolyl tetrazolum(MTT) assay.The expressor gene was determined with SP immunohistochemistry,and image analysis.Results:When Hep-2 cells were treated with CIN for 24 hrs, the cell proliferation was induced and the cell became well differentiated;but when Hep-2 cells was treated for 120 hrs,the cell death occurred by apoptosis.The expression of the oncogenes c-myc protein was markedly reduced,while the change of p53 protein was not obvious.Conclusion:The anti-tumor effect and inducing the differentiation of CIN might be by means of inhibiting the expression of oncogenes and improving the expression of carcinoma suppressor gene.