摘要
从生产实际出发 ,系统论述了羊生产性胚胎移植的组织及技术程序。主要包括 :胚胎移植条件的准备 ;生产场技术助手的培训 ;选择适宜的供、受体羊 ,并加强饲养管理 ;在适宜的季节 ,用宁波激素厂促卵泡素 (FSH) 2 50～ 30 0IU(绵羊 )和 30 0～350IU(山羊 ) ,或用中科院动物所FSH 7.5mg(绵羊 )和 9～ 1 2mg(山羊 ) ,以减量法进行供体超排 ;供、受体比例为 1 :8～ 1 2 ;用炔诺酮阴道栓配合促性腺激素 ,或用氯前列烯醇 (PG)进行受体同期发情处理 ;从供体子宫角手术回收的桑椹胚～早期囊胚 ,移植于受体羊子宫角 ;
According to farm condition, carry out and technique programs of embryo transfer in goats and sheeps were discussed systematically. There are as follows: ①preparing the operation room, the apparatuses and equipments, as well as other articles;②training the working assistants;③selecting the suitable donors and recipients, and careful breeding and management;④in the feasible seasons, superovulation were induced by injections of descending doses of FSH 250～300 IU or 7.5mg(sheeps), or of 300～350 IU or 9～12mg(goats) in donors;⑤the appropriate proportion were 1:8～12 in donors :recipients;⑥estrus synchronization were induced by norgestrel-impregnated intravaginal sponge treatment with injections of FSH, or by injections of estrumate;⑦morulae and early blastocysts collecting from donor's uteri and transferring into uteri of recipients;⑧entire record system.
出处
《草食家畜》
2001年第S2期112-118,共7页
Grass-Feeding Livestock
关键词
胚胎移植
组织实施
技术程序
山羊
绵羊
embryo transfer
carrry out
technique programs
goats
sheeps
