肝癌患者降钙素基因高度甲基化表达的初步探讨

Preliminary Study on Expression of Hypermethylation in the Calitonin Gene in the Patients with Hepatic Cancer

目的 探讨降钙素(Calcitonin,CT)基因高度甲基化在肝癌诊断中的价值。方法 应用多聚酶链聚合反应(PCR)技术检测降钙素基因高度甲基化。结果 6例良性肿瘤全部阴性表达,12例肝癌患者中有9例表达阳性,阳性率为75％。结论 CT基因的高度甲基化在肝癌中有较高的发生率,对肝癌的诊断有一定价值。

Objective To inquire the diagnostic value for hepatic cancer of hypermethylation in the calcitonin gene.Methods To detect hypermethylation in the calcitonin gene by polymerase chain reaction.Results Negative expression in 6 cases with benign tumors and positive expression in 9 cases of 12 with hepatic cancer(75%).Conclusions The results indicated that there was a higher ratio of hypermethylation in the calitonin gene in the patients with hepatic cancer.