摘要
To explore the relationship between genetic variation in coagulation factor Ⅴ and the occurrence of coronary arterial disease (CAD) Methods Unrelated 86 patients with CAD and 102 healthy controls were analyzed by polymerase chain reaction denaturing gradient gel electrophoresis (PCR DGGE) to detect variations in the entire twenty five exons of the factor Ⅴ gene Results Polymorphisms in exon 4 [642 GT (Ser156)], exon 10 [1628 GA (Arg485Lys)], exon 13 [4070 AG (His1299Arg)] and exon 16 [5380 GA (Val1736Met)] were documented The study also identified a novel polymorphism in exon 2 (327 AG) which did not result in amino acid residue substitution The Leiden mutation (Arg506Gln) was not detected in any of our 188 subjects Among the 5 polymorphisms, the allele frequency of 1628 GA was significantly different between CAD patients and controls (0 69 vs 0 81, χ 2=6 908, P <0 01) This is the first report of this finding in a Chinese population Conclusion 1628 GA polymorphism is associated with CAD and it may be a risk factor for CAD morbidity in the Chinese population
To explore the relationship between genetic variation in coagulation factor Ⅴ and the occurrence of coronary arterial disease (CAD) Methods Unrelated 86 patients with CAD and 102 healthy controls were analyzed by polymerase chain reaction denaturing gradient gel electrophoresis (PCR DGGE) to detect variations in the entire twenty five exons of the factor Ⅴ gene Results Polymorphisms in exon 4 [642 GT (Ser156)], exon 10 [1628 GA (Arg485Lys)], exon 13 [4070 AG (His1299Arg)] and exon 16 [5380 GA (Val1736Met)] were documented The study also identified a novel polymorphism in exon 2 (327 AG) which did not result in amino acid residue substitution The Leiden mutation (Arg506Gln) was not detected in any of our 188 subjects Among the 5 polymorphisms, the allele frequency of 1628 GA was significantly different between CAD patients and controls (0 69 vs 0 81, χ 2=6 908, P <0 01) This is the first report of this finding in a Chinese population Conclusion 1628 GA polymorphism is associated with CAD and it may be a risk factor for CAD morbidity in the Chinese population
基金
ThisstudywassupportedbytheScientificandTechnologicalCommitteeofShanghai (No 9741 1 90 0 3)
