摘要
Objective To evaluate the function of T cells in chronic myeloid leukemia (CML) Methods Interleukin 1β (IL 1β), interleukin 2 (IL 2), interleukin 3 (IL 3), interleukin 4 (IL 4), interleukin 6 (IL 6), tumor necrosis factor alpha(TNFα), and granulocyte macrophage colony stimulating factor (GM CSF) gene expressions were investigated by reverse transcription polymerase chain reaction (RT PCR) assay in fluorescence active cell sorter (FACS) sorted peripheral blood CD2+/CD56 T cells from 12 CML patients, 10 meylodysplastic syndrome (MDS) patients and 7 normal individuals Results TNFα mRNA was transcribed in T cells from all of the CML, MDS and normal individuals IL 1β mRNA was transcribed in T cells from 10 CML, 9 MDS and 6 normal individuals Low levels of IL 2 and IL 4 mRNA were detected in 5 CML patients IL 3, IL 6 and GM CSF mRNA were undetectable in all samples Conclusion IL 4 and IL 2 were expressed abnormally in T cells of CML
Objective To evaluate the function of T cells in chronic myeloid leukemia (CML) Methods Interleukin 1β (IL 1β), interleukin 2 (IL 2), interleukin 3 (IL 3), interleukin 4 (IL 4), interleukin 6 (IL 6), tumor necrosis factor alpha(TNFα), and granulocyte macrophage colony stimulating factor (GM CSF) gene expressions were investigated by reverse transcription polymerase chain reaction (RT PCR) assay in fluorescence active cell sorter (FACS) sorted peripheral blood CD2+/CD56 T cells from 12 CML patients, 10 meylodysplastic syndrome (MDS) patients and 7 normal individuals Results TNFα mRNA was transcribed in T cells from all of the CML, MDS and normal individuals IL 1β mRNA was transcribed in T cells from 10 CML, 9 MDS and 6 normal individuals Low levels of IL 2 and IL 4 mRNA were detected in 5 CML patients IL 3, IL 6 and GM CSF mRNA were undetectable in all samples Conclusion IL 4 and IL 2 were expressed abnormally in T cells of CML
