摘要
The conformation of Calmodulin(CaM) induced by lanthanides has been examined using fluorescence methods. With the addition of lanthanide (Ln 3+ ), the intrinsic fluorescence intensity of CaM without calcium ions (Apo CaM) first increases and then decreases. Ln 3+ causes the decrease of intrinsic fluorescence intensity of calcium saturated CaM (Ca 2+ 4 CaM) only at high concentrations. At low concentrations, Ln 3+ results not only in the enhancement of fluorescence intensity of Apo CaM, but also in a blue shift of the maximum emission wavelengh of dansyl labeled calmodulin(Apo D CaM). The molecular mechanism of the interaction between Ln 3+ and CaM has been discussed in the light of the fluorescence spectra.
The conformation of Calmodulin(CaM) induced by lanthanides has been examined using fluorescence methods. With the addition of lanthanide (Ln 3+ ), the intrinsic fluorescence intensity of CaM without calcium ions (Apo CaM) first increases and then decreases. Ln 3+ causes the decrease of intrinsic fluorescence intensity of calcium saturated CaM (Ca 2+ 4 CaM) only at high concentrations. At low concentrations, Ln 3+ results not only in the enhancement of fluorescence intensity of Apo CaM, but also in a blue shift of the maximum emission wavelengh of dansyl labeled calmodulin(Apo D CaM). The molecular mechanism of the interaction between Ln 3+ and CaM has been discussed in the light of the fluorescence spectra.
基金
the National Natural Science Foundation of China! (No . 39370 1 80 )
