血小板活化因子受体在人皮肤角质形成细胞中的表达及其调控

Expression and regulation of platelet-activating factor receptor in human keratinocytes

目的?观察血小板活化因子受体(PAF-R)在正常人皮肤角质形成细胞中的表达调控特征。方法:应用免疫组织化学 法检测PAF-R在正常皮肤组织及HaCaT细胞中的表达,采用RT-PCR法检测PAF-R在HaCaT细胞基因水平的表达特征, 并用流式细胞术定量检测刺激因素作用下HaCaT细胞中PAF-R的表达调控情况。结果:PAF-R在正常人角质形成细胞中 有强烈的表达,阳性部位位于细胞膜及细胞质。PAF-R在HaCaT细胞转录水平有表达,且组织型mRNA产物高于白细胞型 mRNA产物。PAF-R在HaCaT细胞膜内外均有阳性表达,细胞内表达量约是膜表达量的4.82倍。IFN-γ、维甲酸可以上调 PAF-R在HaCaT细胞的膜表达量。结论:皮肤角质形成细胞在细胞、蛋白及基因水平均有PAF-R强烈表达,而且这种表达 可被炎症因子调控,提示PAF系统可能在皮肤炎症中起重要作用。

Objective:To investigate the expression and regulation of the platelet-activating factor receptor(PAF-R)in human keratinocytes.Methods:The expression of PAF-R in skin tissues and HaCaT cells was examined by immunohis-tochemistry.The expression of PAF-R in HaCaT cell mRNA level was detected by RT-PCR.The expression and regulation of PAF-R were measured by flow cytometry in HaCaT cells treated with various stimuli.Results:The expression level of PAF- R was very high in human keratinocytes,mainly located on cell membranes and cytoplasma.PAF-R also expressed on HaCaT cells transcription levels,and the tissue-type PAF-R mRNA was more than that of leucocyte-type PAF-R mRNA.PAF-R ex-pressed on both membrane and intracellar part of HaCaT cell,and the intracellular expression was about4.82times that of membrane expression.IFN-γand retinoic acid upregulated the membrane expression of PAF-R in HaCaT cell.Conclusion:Human keratinocytes can highly express PAF-R at cell,protein and transcription levels,and the expression characteristic can be regulated by some inflammatory factors,which indicates the PAF system may play an important role in the skin inflamma-tion. [