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血小板衍生生长因子诱导人血管平滑肌细胞增殖时钙调素的变化 被引量：5

Effects of platelet-derived growth factor BB on proliferation and calmodulin content in human vascular smooth muscle cells
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摘要 目的?观察血小板衍生生长因子(PDGF)对培养的人血管平滑肌细胞增殖及钙调素含量变化的作用。方法:应用3H- TdR掺入法和磷酸二酯酶法,观察不同浓度(1、10、20、30、40ng/ml)PDGF-BB对培养的人血管平滑肌细胞DNA合成和钙调素 含量变化的影响及其时间效应。结果:不同浓度PDGF-BB作用后,处于静止状态的人血管平滑肌细胞DNA的合成增加,并呈 现明显的浓度依赖关系,在40ng/ml浓度时DNA的合成达到高峰(P<0.01)。平滑肌细胞在进入细胞增殖周期后,细胞内钙调 素含量逐渐增加,在9h时出现一个短暂的高峰,随后逐渐下降;不同浓度PDGF-BB作用后,钙调素含量增加,并呈现出明显的 浓度依赖关系,30ng/ml浓度时作用最为显著(P<0.01)。结论:PDGF可促进培养的人血管平滑肌细胞增殖,该作用可能与增 加钙调素含量有关。 Objective:To investigate the effects of platelet-derived growth factor BB(PDGF-BB)on proliferation and calmodulin content in human vascular smooth muscle cells(hVSMCs).Methods:The hVSMCs were cultured;the effects of PDGF-BB on DNA synthesis and calmodulin content in hVSMCs were assayed at different time points and different concentra-tions by 3 H-TdR incorporation and phosphodiesterase in vitro.Results:The DNA synthesis of quiescent hVSMCs was pro-moted in a dose-dependent manner,with the maximal response at a PGDF-BB concentration of40ng/ml at36h.Calmodulin level was increased after hVSMCs proliferation and reached a peak at9h.The content of calmodulin increased in a dose-de-pendent manner,with the maximal response at a PGDF-BB concentration of30ng/ml.Conclusion:PDGF-BB can promote the proliferation of hVSMCs,which may be related to the calmodulin level. [
作者 任雨笙 杜荣增 黄佐 潘晓明 樊民 吴宗贵
机构地区 第二军医大学长征医院心血管内科
出处 《第二军医大学学报》 CAS CSCD 北大核心 2004年第6期620-622,共3页 Academic Journal of Second Military Medical University
基金 上海市科技发展攻关计划基金(014119072) 上海市自然科学基金(03ZR14029).
关键词 血小板衍生生长因子 诱导 血管平滑肌细胞 细胞增殖 钙调素 platelet-derived growth factor vascular smooth muscle cells cell proliferation cell cycles calmodulin
分类号 R392.2 [医药卫生—免疫学]
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参考文献5

  • 1[1]Rasmussen CD, Lu KP, Means RL,et al. Calmodulin and cell cycle control[J]. J Physiol Paris,1992,86(1) :83-88.
  • 2[2]Hao X, Mansson-Broberg A, Gustafsson T, et al. Angiogenic effects of dual gene transfer of bFGF and PDGF-BB after myocardial infarction [J]. Biochem Biophys Res Commun, 2004,315(4): 1058-1063.
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同被引文献20

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引证文献5

二级引证文献14

第二军医大学学报
2004年 第6期

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