摘要
ＩｎｔｒｏｄｕｃｔｉｏｎＳｉｎｃｅｔｈｅｌａｓｔｔｗｅｎｔｙｙｅａｒｓ，ｈｙｄｒｏｐｈｏｂｉｃｉｎｔｅｒａｃｔｉｏｎｃｈｒｏｍａｔｏｇｒａｐｈｙ（ＨＩＣ）ｔｅｃｈｎｉｑｕｅｈａｓｂｅｅｎｓｕｃｃｅｓｓｆｕｌｙａｐｐｌｉｅｄｔｏｐｕｒｉｆｙｉｎｇｍａｎ...
A novel hydrophobic medium with propyl as functional group and Sepharose 6B as matrix was designed and synthesized. The comparison of the hydrophobic medium synthesized with the commercial products was made by hydrophobic interaction chromatography(HIC) in isolating recombinant hepatitis B surface antigen(r HBsAg). r HBsAg was further purified to the final products by following a downstream procedure . The results indicate that the synthesized hydrophobic medium possesses a stable structure and desired physical and chemical properties. They were used to purify r HBsAg with a high yield and purity. Both the immunity and stability of hepatitis vaccine made by the r HBsAg products have reached the same level as other similar kinds of products.
