血管内皮生长因子mRNA在食管癌中的表达和临床意义

Expression and clinical significance of vascular endothelial growth factor in esophegeal carcinoma

目的 检测和分析食管癌中血管内皮生长因子 (VEGF)mRNA的表达情况 ,探讨其与食管癌的淋巴结转移、临床分期的关系。方法 应用逆转录—聚合酶链式反应 (RT -PCR)检测和分析 3 6例食管癌组织和 2 4例癌旁 6cm以上的正常食管组织中VEGFmRNA的表达情况。结果 ( 1)VEGF189mRNA的表达与食管癌 :在 3 6例癌组织中 ,有 2 2例表达VEGF189mRNA ( 61.1% )。在 2 4例癌旁正常组织中 ,有 1例表达VEGF189mRNA( 4 .2 % )。 ( 2 )VEGFmRNA的表达与淋巴结转移及TNM分期 :在 18例有淋巴结转移的病例中VEGF189mRNA表达 16例 ( 88.9% ) ;在18例无淋巴结转移的病例中VEGF189mRNA表达 6例 ( 3 3 .3 % )。Ⅰ期中VEGF189mRNA表达 3例( 2 1.4% ) ;Ⅱ期中VEGF189mRNA表达 11例 ( 84.6% ) ;Ⅲ期中VEGF189mRNA表达 8例 ( 88.9% )。结论 VEGF189mRNA与食管癌的侵袭。

Objective To examine and analyze the expression of vascular endothelial growth factor (VEGF) mRNA in esophageal carcinoma and the relationship among VEGF,lymph node metastasis and the clinical stage.Methods The expression of VEGF was detected in 36 patients with esophageal carcinoma and 24 surrounding esophageal tissues 6 cm away from the cancer tissues by reverse transcription polymerase chain reaction (RT-PCR).Results In 36 esophageal carcinoma tissues,22(61.1%) cases expressed VEGF 189 mRNA,while one (4.2%) expressed VEGF189mRNA out of 24 paracancer esophageal tissues.The expression level of VEGF189mRNA was higher in the patients with lymph node metastasis (16/18,88.9%) than those without lymph node metastasis (6/18,33.3%,P<0.01).VEGF189mRNA was expressed in 21.4%,84.6% and 88.9% cases of TNM stage Ⅰ,Ⅱ and Ⅲ respectively.Conclusion The expression of VEGF 189 is significantly associated with the behavior of invasion and metastasis of esophageal carcinoma.