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抗HCV NS3抗原单克隆抗体的研制与初步鉴 被引量:1

Preparation of monoclonal antibodies against HCV NS3 antigen and their characteristic study
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摘要 目的:为研制抗丙型肝炎病毒(HCV)NS3抗原单克隆抗体,用于检测丙肝患者血清中的HCVNS3抗原。方法:用基因工程表达的HCV非结构区NS3蛋白与鼠血清白蛋白交联后,免疫BALB/c小鼠,用杂交瘤技术获得能分泌单克隆抗体(McAb)的细胞株。结果:获得有实用价值的两株McAb。两株McAb与免疫抗原有较强的抗原-抗体反应,与HCVNS4、NS5及核心区C33肽、CP9、CP10抗原无反应性,在竞争ELISA中,对HCV-IgG阳性血清有较好的抑制作用。结论:用基因工程表达的HCV非结构区NS3蛋白,能够制备出有实用价值的单克隆抗体。 Objectives:]To prepare BALB/c mice monoclonal antibodies against HCV NS3 antigen and to detect HCV NS3 protein in hepatitis C patients sera. WTHX Methods: BALB/c mice were immunized with HCV NS3 antigen which was expressed by gene cloning and coupled with murine serum albumin,and the monoclonal antibodies were obtained by hybridoma technique. Results: Two monoclonal antibodies( 2B8, 6D6) were obtained.The reactions of the 2 McAbs were strong with the immunized antigen NS3,but no reactivities were detectable with HCV NS4,NS5 and its core antigen C33 peptide,CP9 and CP10.The 2 McAbs could inhibit antiHCVIgG positive serum in the competitive ELISA. Conclusion: From gene cloning HCV NS3,two monoclona antibodies against HCV NS3 protein were prepared with practical value.
出处 《医学研究生学报》 CAS 1999年第3期48-50,共3页 Journal of Medical Postgraduates
关键词 丙型肝炎 NS3 单克隆抗体 Hepatitis C NS3 Monoclonal antibody
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