RP-HPLC法同时测定大黄及其他样品中的五种游离蒽醌含量

Determination of five antraquinones in chinese Rhubuarbs and other samples

目的：测定大黄及其他样品中的五种游离蒽醌（芦荟大黄素、大黄酸、大黄素、大黄酚和大黄素甲醚）的含量。方法：采用反相高效液相色谱（ＲＰ－ＨＰＬＣ）法以甲醇、水、氯仿和冰醋酸混合液（５００∶８０∶２５∶２，ｖ／ｖ）为流动相，色谱柱采用ＹＷＧ－Ｃ１８不锈钢柱，紫外２５４ｎｍ波长检测。样品经乙醚处理挥干后流动相溶解进样测定，１２ｍｉｎ可完成一次测定过程。结果与结论：结果准确、灵敏，回收率高，是适用于药品质量监测、优化有效成分的提取方法。

Objectives: To determine five antraquinones(Aloeemodin, Rhein, Emodin, Chrysophanol,Physcion) in chinese Rhubuarbs and other samples by RPHPLC with a simple and rapid method. Methods: The analytical column was packed with YWGC18 .The mobile phase consisted of methanolwaterchloroformacetic acid(50080252,v/v),and detected by UV detector at a wavelength of 254nm. The sample was injected directly into the HPLC system after soluted with mobile phase. Results: Using this method,Chinese Rhubuarbs, Polygonum multiflorum, Xin Baoshen Pian and extract of Chinese Rhubarbs were determined. Conclusions: This method is accurate,sensitive and has high takeback rate.