糖尿病病人血浆凝血因子Ⅶ的测定与临床意义

Detection of plasma coagulation factor YD in patients with diabetes mellitus and its clinical significance

目的:测定20例NIDDM糖尿病病人血浆凝血因子Ⅶ的水平,探讨其临床意义。方法:FⅦa测定采用重组可溶性组织因子法;FⅦ:Ag测定采用ELISA法;FⅦ:C采用一阶段凝固法。并计算FⅦa/FⅦ:Ag与FⅦ:C/FⅦ:Ag的相对比值。结果:患者FⅦa为2.8±1.0mg/ml,FⅦ:C为109±20％,FⅦ:Ag为75±18％,FⅦa/FⅦ:A为3.73,FⅦ:C/FⅦ:Ag为1.45,与对照组两组(健康成人、健康老年人)比较,除FⅦ:Ag外,其余数据均有增高差异呈显著性(P<0.01,P<0.05)。结论:研究表明糖尿病患者具有高凝状态,应采取防止血栓形成的措施。

Objective To detect plasma Level of FⅦ in 20 cases patients with Insulin nondependent diabetes mellitus and discuss its clinical significance.Method: FⅦ was detected with recombinant soluble tissue factor method. FⅦ: Ag was detected with ELJSA. Phase 1 coagulation method was used to detect FⅦ: C. Then calculate relative value of FⅦa/FⅦ: Ag and FⅦ: C/FⅦ: Ag. Results: FⅦ a level was 2.8±1.0 (ng/ml).FⅦ: C was 109±20% . FⅦ a/FⅦ : Ag value was 3.73 and FⅦ : C/FⅦ: Ag value was 1.45 Besides FⅦ : Ag, the others were significantly higher than those of two group controls (healthy adults group, healthy old man or woman group( P<0.01) . Conclusion:The results of this study showed that the patients with diabetes mellitus were hyper- coagulation state. Therefore, we should take meausres to combat the thrombosis.