含有CD基因的腺病毒载体的构建

Construction of Adenovirus Vectors Carrying CD Gene and LacZ Gene

目的 :构建携带CD基因和LacZ报告基因的腺病毒载体。方法 :先将真核表达载体质粒 pCI中的转录框架构建入腺病毒载体中 ,后再将CD和LacZ基因分别装配到框架的多克隆位点内。结果 :经过酶切鉴定成功地构建了分别携带CD基因和LacZ报告基因的腺病毒载体 ,对肿瘤的前药转换基因治疗有重要意义。

Objective: To construct adenovirus vectors carrying CD gene and LacZ gene. Methods: The open reading frame was released from eukaryonic expression vector plasmid pCI and inserted into the adenovirus shuttle vectors p△E 1 SP1A. Then CD and LacZ genes were put into the polylinker site of the open reading frame in the modified adenovirus vectors respectively. Results: The structure of the adenovirus vectors carrying CD gene and LacZ gene was identified by restriction enzyme analysis. The newly constructed adenovirus is useful for prodrug transformation gene therapy.