潜艇常见气体对离体细胞化学发光的影响

Effects of Common Gases in Submarines on Chemiluminescence of in vitro Pulmonary Alveolus Macrophage

目的 :用化学发光法检测O2 、CO2 、CO和正庚烷 (CxHy)四种气体对离体家兔肺泡巨噬细胞 (AM )的直接效应。 方法 :将AM悬液暴露于上述气体 ,不同时间检测用PMA激发的细胞发光及其存活率。结果 :培养在 99.5 %N2 (0 .5 %O2 )中的AM受激发光仅能维持 10h ;高浓度O2 、CO2 、CO和CxHy具有刺激或增强AM发光之效应 ;含 16 %O2 、1.9%CO2 、46mg/m3 CO和 176mg/m3 CxHy的模拟潜艇混合气体未见对AM激发光功能和存活率产生不利影响。结论 :空气氧含量对细胞十分重要 ,提高氧浓度有利于细胞存活 ,但氧浓度 >16 %和 <2 0 %的低氧还原性气体环境更有利细胞长时间生存。

Objective: To examine the direct effects of four gases-O 2,CO 2,CO and heptane (CxHy) on chemiluminescence of in vitro alveolus macrophage(AM) from rabbits with a luminescence technique. Methods: The AM suspension was exposed to the above mentioned gases and the chemiluminescence of AM stimulated with phorbol myristate acetate(PMA) was examined by chemiluminometry at different times. The survival rate of AM was counted with a microscope.Results: In the air with 0.5% O 2(99.5%N 2), the excited chemiluminescence of AM was only maintained for 10 hours, and with high concentrations of O 2, CO or CxHy, the excited chemiluminescences of AMs showed a transient enhancing effect. It was found that in the mixed air with 16%O 2,1.9%CO 2, 46mg/m 3 CO and 176mg/m 3 heptane, which simulated the submarine condition, neither the luminescence function nor the viability of AM was impaired. Conclusion:O 2 in the air is very important for in vitro cells. High concentration of O 2 is beneficial to cell survival, but the reducing gas environment with low O 2 concentration (>16% and <20%) is more favourable for prolonged living of cells.