原位杂交法检测喉鳞癌组织中人乳头状瘤病毒的研究

6 Detection of human papillomavirus DNA in laryngeal squamous cell carcinomas by in situ hybridization

目的探讨喉鳞状细胞癌与人乳头状瘤病毒（ＨＰＶ）亚型的关系。方法采用原位杂交技术，地高辛标记Ｈｐｖ６Ｂ／１１、１６和１８型ＤＮＡ作探针，对３２例喉鳞癌石蜡包埋标本中Ｈｐｖ－ＤＮＡ同源序列进行了检测。结果Ｈｐｖ６Ｂ／１１型杂交在喉鳞癌中阴性；Ｈｐｖ１６型杂交阳性在喉鳞癌为１４／３２（４６％）；Ｈｐｖ１８型杂交阳性在喉鳞癌为９／３２（２８％）。各型表达与肿瘤分化程度无关（Ｐ＞０．０５）。结论Ｈｐｖ１６和Ｈｐｖ１８型感染与喉鳞癌的发生有密切关系。

Objective:To study the relationship between human papillomavirus(HPV) genomes 6B/11,16,18 DNA and the carcinogenesis of laryngeal carcinoas(LSOC).Method:Using in situ hybridization(ISH),32 cases of LSCC had been fixed in formalin and embedded in paraffin,then HPV genome 6B/11,16 and 18 DNA were examined.Results:All cases were HPV genomes 6B/11 negative;14(46%)cases were HPV genome 16 positive;9(28%)cases were HPV genome 18 positive.Conclusion:These result suggest that HPV genomes 16,18 infetion might be closely associated with the development of LSCC. [