CD44v6、nm23-H1在乳腺癌中的表达及与淋巴结转移的关系

7 The relationship between the expression of CD44v6,nm23 H1 protein and lymphatic metastasis of breast carcinoma

目的探讨ＣＤ４４ｖ６、ｎｍ２３－Ｈ１基因蛋白与乳腺癌淋巴结转移的关系。方法应用免疫组织化学ＳＰ法检测４６例乳腺癌组织中ＣＤ４４ｖ６，ｎｍ２３－Ｈ１基因蛋白的表达。结果ＣＤ４４ｖ６阳性检出率为６９．６％（３２／４６），其中淋巴结受累乳腺癌阳性率为７２％（１８／２５），无淋巴结转移组检出率为６６．７％（１４／２１），两者比较差异无显著性（Ｐ＞０．０５），但淋巴结受累乳腺癌的ＣＤ４４ｖ６强阳性表达检出率为６４％（１６／２５），明显高于淋巴结未受累乳腺癌的强阳性表达检出率３３．３％（７／２１）（Ｐ＜０．０５）；ｎｍ２１－Ｈ１阳性表达率为７１．７％（３３／４６），其中淋巴结受累乳腺癌和未受累乳腺癌中的阳性检出率分别为８０％（２０／２５）、６１．９％（１３／２１），前者略高于后者，但差异无显著性（Ｐ＞０．０５）。结论ＣＤ４４ｖ６、ｎｍ２３－Ｈ１异常表达可能是乳腺癌发生、发展的重要分子学改变，ＣＤ４４ｖ６强表达可能对判断乳腺癌转移风险有一定指导意义。

Objective:To investigate the relationship between CD44v6,nm23 H1 expression to lymphatic metastasis of breast carcinoma.Methods:The SP immunohistochemical method was used to study the expression of CD44v6 and nm23 H1 protein in 46 cases of breast carcinoma.Result:Expression of CD44v6 was detected in 69 6%(32/46)of the cases studied,Among them,18 cases(72%,18/25)with lymphatic metastasis were CD44v6 positive;14 cases (66 7%,14/21)without lymphatic metastasis were CD44v6 positive;Compare to lymphatic metastasis group,the difference was no significant(P>0 05).However high experssion of CD44v6 in group with lymphatic metastasis(64%) was significat higher than in group without lymphatic metastasis(33 3%)(P<0 05);Expression of nm23 H1 was detected in 71 7%(33/46)of the cases studied.20 cases (80%,20/25)with lymphatic metastasis were nm23 H1 positive 13 cases(61 9%,13/21)without lymphatic metastasis were nm23 H1 positive,Compare to lymphatic metastasis group,the difference was no significant(P>0 05).Conclusion:The abnormal expression of CD44v6 and nm23 H1 may be associated with the carcinogensis and development of breast carcinoma.The high expression of CD44v6 may play a crucial role in predicting metastasis of breast carcinoma,however the expression of nm23 H1 may have no crooelation with lymphatic metastasis of breast carcinoma. [