烧伤患者人巨细胞病毒感染状况的研究

Study of human Cytomegalovirus infection status in burned patients

目的：了解烧伤患者人巨细胞病毒感染状况。方法：采用聚合酶链反应（ＰＣＲ）和ＥＬＩＳＡ法分别对１４５例烧伤患者尿中人巨细胞病毒ＤＮＡ（ＨＣＭＶＤＮＡ）和血清中ＨＣＭＶ－ＩｇＭ抗体进行检测。结果：烧伤患者ＨＣＭＶＤＮＡ阳性率为６１．３８％，正常对照组为２９．４９％，差异有显著性（Ｐ＜０．０１）；烧伤总面积（ＴＢＳＡ）≥３０％患者阳性率为７３．１３％，显著高于ＴＢＳＡ＜３０％患者５１．２８％（Ｐ＜０．０５）；小儿烧伤组阳性率为７８．７９％显著高于成人烧伤组５６．２５％（Ｐ＜０．０５）。同时，烧伤患者血清中ＨＣＭＶ－ＩｇＭ抗体阳性率为１０．３４％，在ＰＣＲ检测阳性８９例患者中有１５例ＨＣＭＶ－ＩｇＭ为阳性。结论：烧伤后人巨细胞病毒感染率显著升高，尤其以大面积烧伤患者更甚，在临床上应引起广泛重视。

Objective:To investigate the status of human cytomegalovirus infection in burned patients.Methods:Polymerase chainreaction(PCR) and enzymelinked immunosorbent assay (ELISA) were used to detect HCMV DNA from urine and serum anti-HCMV-IgM in 145 patients with burn and in 156 controls. Results: The HCMV DNA positive rate detected by PCR in the burned patients was 61.38% and 29.19% in the controls, there was a striking difference ( P < 0.01 ) between the two groups. The HCMV DNA positive rate was 73.13% in patients of TBSA≥30%, which was evidently higher than in those of TBSA<30%. The positive rate was 78.79% and 56.25% respectively in the patients of children and adults. In 89 PCR positive cases, 15 cases showed anti-HCMV-IgM postitive.Conclusion:The result indicated that the burned patients were the group with high HCMV infection rate. [