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培养的海马神经元致痫后谷氨酸的合成和释放的变化

THE CHANGES OF GLUTAMATE SYNTHETISED AND RELEASED IN CULTURED HIPPOCAMPAL NEURONS AFTER EPILEPTIC INDUCED
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摘要 通过谷氨酸(Glutamate,Glu)免疫细胞化学染色法观察到马桑内酯(CoriariaLactone,CL)(2.5×10-5mol/L)作用于体外培养的海马神经元6h呈色增强,此后呈色反应明显减弱,阳性细胞数减少,胞体变小,突起短而稀少。MK-801(4×10-5mol/L)可降低CL引起的海马神经元Glu免疫反应性。高效液相色谱法(HPLC)测定CL作用于培养的海马神经元24h后培养基中Glu和天门冬氨酸(Asp)含量增加(P<0.001),MK-801并不能阻断此种效应。结果提示CL致痫后,早期神经元内Glu合成增加,后期向胞外释放。 The hippocampal neurons in culture wre immunocytochemically stained with anti glutamate serum. The positive neurons stained strongly 6h after CL administration (2 5×10 -5 mol/L). The number of positive neurons decreased with smaller cell bodies and shorter, thicker processes. MK 801(4×10 -5 mol/L) partly weakened the CL induced Glu immunoreactivity of hippocampal neurons. With reversed phase high performance liquid chromatography (HPLC), glutamate and aspartate of culture media significantly increased after hippocampal neurons had been challenged with CL for 24h ( P <0 001). MK 801 did not block the above changes. These results suggest that the epileptic hippocampal neurons induced by CL produce more glutamae in earlier stage, and later release it to outside of neurons.
出处 《中国组织化学与细胞化学杂志》 CAS CSCD 1998年第4期94-98,共5页 Chinese Journal of Histochemistry and Cytochemistry
基金 国家自然科学基金
关键词 海马 细胞培养 谷氨酸 癫痫 Hippocampus Cell culture Glutamate Epilepsy
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