人脊髓前角运动神经元190KD蛋白的初步研究

A PRELIMIARY STUDY OF A 190KD PROTEIN OF MOTONEURONS IN ANTERIOR HORN OF HUMAN SPINAL CORD

本研究采用ＳＤＳ凝胶电泳方法从人脊神经前根中分离出人脊髓前角运动神经元特有的蛋白—１９０ＫＤ。将该蛋白作为抗原，免疫ＢＡＬＢ／ｃ小鼠，经杂交瘤技术，获得了抗１９０ＫＤ蛋白的单克隆抗体。免疫细胞化学检测表明，１９０ＫＤ单抗与脊髓灰质前角神经元、前根及肌支发生阳性反应。实验结果提示，１９０ＫＤ蛋白分布在脊髓运动神经元胞体及脊神经的前根和肌支纤维中。

A 190KD protein of motoneurons was isolated from the anterior roots of human spinal cord by SDS PAGE. The monoclonal antibody was prepared through immunizing the BALB/c mouse with 190KD protein. The results of the immunohistochemistry showed that positive products distributed in motoneurons in anterior horn of spinal cord, the fibers of anterior roots, and muscular branches of peripheral nerve. This study suggests that the 190KD protein may be a specific protein for motoneurons, which exists only in the motoneurons, anterior roots and muscular branches.