摘要
Objective: To investigate multi gene expression in the highly metastasizing human ovarian cancer cell line HO 8910PM and its mother cell line HO 8910. Method: The expression of 9 kinds of gene products in HO 8910PM and its mother cell line HO 8910 was detected by S P immunohistochemical method. Result: Eight kinds oncogene products showed various degrees of positive expression in both HO 8910PM and HO 8910 cell lines except gene bax. The expression of P53, Cyclin D 1, CD 44 ν6 and EGFR in HO 8910PM was stronger than that in HO 8910. However, the expression of P16, nm23 in HO 8910PM was weaker than that in HO 8910. There was no significant difference on the expression of C erb B 2 and bcl 2 between the two cell lines. Conclusion: Stronger invasive and metastatic patential is found in HO 8910PM than that in HO 8910. Carcinogenesis is a result of multi oncogene and multiple step process cooperation.
Objective: To investigate multi gene expression in the highly metastasizing human ovarian cancer cell line HO 8910PM and its mother cell line HO 8910. Method: The expression of 9 kinds of gene products in HO 8910PM and its mother cell line HO 8910 was detected by S P immunohistochemical method. Result: Eight kinds oncogene products showed various degrees of positive expression in both HO 8910PM and HO 8910 cell lines except gene bax. The expression of P53, Cyclin D 1, CD 44 ν6 and EGFR in HO 8910PM was stronger than that in HO 8910. However, the expression of P16, nm23 in HO 8910PM was weaker than that in HO 8910. There was no significant difference on the expression of C erb B 2 and bcl 2 between the two cell lines. Conclusion: Stronger invasive and metastatic patential is found in HO 8910PM than that in HO 8910. Carcinogenesis is a result of multi oncogene and multiple step process cooperation.
