摘要
This work was supported by a grant from the National Natural Science Foundation of China (No.39730420). This is one of papers of the special issue on gene therapy research (Chin J Cancer Res Vol. 9 No. 4 December, 1997). Human IL 6 gene was transduced into FBL 3 murine erythroleukemia cells in vitro by calcium phos phate co participation. After selection in the presence of G418, limiting dilution and biological activity assay, G418 resistant clone that secreted the highest level of IL 6 (225.6 U/ml) was selected out of 24 IL 6 secreting clones. The FBL 3 cells secreting the highest level of IL 6 (FBL 3 IL 6) showed decreased growth potential and clono genicity in vitro. Inhibition of cell growth and clone formation was found to be closely related to the level of IL 6 secretion. FBL 3 IL 6 cells grew more slowly than wild type FBL 3 leukemia cells and FBL 3 cells secreting lower level of IL 6 (21.3 U/ml) when inoculated s.c. into C57BL/6 mice. The mice inoculated with FBL 3 IL 6 cells showed prolonged survival period than those inoculated with control leukemia cells. Increased cytotoxic activities of splenic NK and CTL were found in mice inoculated with FBL 3 IL 6 cells. The secretions of IL 2, TNF and GM CSF from murine splenocytes were also found to be greatly elevated after the inoculation of FBL 3 IL 6 leukemia cells. These data suggested that transduction of IL 6 gene into FBL 3 cells magnificently decreased the tumorigenicity and increased the immunogenicity of the leukemia cells, could induce specific and nonspecific antitumor immune responses. IL 6 gene modified leukemia cells might be of great interests to be used as vaccine for the treatment of leukemia.
This work was supported by a grant from the National Natural Science Foundation of China (No.39730420). This is one of papers of the special issue on gene therapy research (Chin J Cancer Res Vol. 9 No. 4 December, 1997). Human IL 6 gene was transduced into FBL 3 murine erythroleukemia cells in vitro by calcium phos phate co participation. After selection in the presence of G418, limiting dilution and biological activity assay, G418 resistant clone that secreted the highest level of IL 6 (225.6 U/ml) was selected out of 24 IL 6 secreting clones. The FBL 3 cells secreting the highest level of IL 6 (FBL 3 IL 6) showed decreased growth potential and clono genicity in vitro. Inhibition of cell growth and clone formation was found to be closely related to the level of IL 6 secretion. FBL 3 IL 6 cells grew more slowly than wild type FBL 3 leukemia cells and FBL 3 cells secreting lower level of IL 6 (21.3 U/ml) when inoculated s.c. into C57BL/6 mice. The mice inoculated with FBL 3 IL 6 cells showed prolonged survival period than those inoculated with control leukemia cells. Increased cytotoxic activities of splenic NK and CTL were found in mice inoculated with FBL 3 IL 6 cells. The secretions of IL 2, TNF and GM CSF from murine splenocytes were also found to be greatly elevated after the inoculation of FBL 3 IL 6 leukemia cells. These data suggested that transduction of IL 6 gene into FBL 3 cells magnificently decreased the tumorigenicity and increased the immunogenicity of the leukemia cells, could induce specific and nonspecific antitumor immune responses. IL 6 gene modified leukemia cells might be of great interests to be used as vaccine for the treatment of leukemia.
