妊高征母血、脐血染色体稳定性和内皮素变化的研究

Study on Blood Chromosome Instability and ET in Maternal and Umbilical Blood of Women With PIH

目的 在致妊高征(PIH)众多因素中,观察PIH孕妇外用血及其脐血姐妹染色单体互换(SCE)频率和内皮素(ET)的变化及其两者的关系,以资探讨PIH的病因和提高PIH的监测水平。方法 采用SCE法和放免法分别对21例PIH病人和15例正常孕妇(对照组)的外周血及其所分娩新生儿脐血SCE频率及其ET进行测定。结果 PIH组外周血和脐血SCE频率分别为6.11±1.29,5.98±1.38;对照组外周血和脐血SCE频率分别为3.45±O.71,3.16±0.57,其两组比较,差异有极显著性(P<0.001)。PIH组外用血和脐血SCE频率皆异常。PIH组外用血ET含量的均值(51.87±24.72pg/ml)明显低于其脐血的均值(63.65±27.63pg/ml),两者比较差异有显著性(P<0.05)。两组SCE和ET相关性分析无明显相关性(r=0.092,P>0.05)。结论 PIH与遗传物质DNA损伤有关,SCE频率可作为一项诊断、预测PIH的重要指标。PIH孕妇脐血ET增高有助于PIH发病机理的揭示。

Objective To study sister chromatid exchange(SCE) frequency in lymphocytes an Endothelin(ET) in maternal and umbilical blood of pregnant women with pregnancy induced Hypertensive (PIH) and relation between SCE and ET. It is objective to study causes and best assay about PIH. Methods The frequency of SCE in peripheral blood lymphocytes and Radio immuno assay on ET of 21 pregnant women with PIH and normal pregnant women as well as the umbilical blood of their infants were investigation. Results The mean frequency of SCE in maternal and umbilical blood with normal pregnancy were 3. 45±0. 71 and 3. 16±0. 57 respectively,while they were 6. 11±1. 29 and 5. 98 ± 1. 38 in PIH group respectively. The difference of SCE levels between the groups of mother was statistically significant(P<0. 001),so was that of the umbilical blood group. The mean ET in maternal blood with PIH was 51. 87±42. 72pg/ml,while it was 63. 65±27. 63pg/ml in the umbilical blood group. The difference of ET levels between the 2 blood groups was statistically significant (P<0. 05). SCE and ET in 2 groups were not statistically correlation(r=0. 092,P>0. 05). Conclusion One of the causes of PIH may be relatad to heredity injury. Thus,SCE will be a new diagnostic index to predict PIH. The mean ET in umbilical blood with PIH is increased. It is to help announce on pathogenesis of PIH.