Ferrocene-Nafion修饰厚膜碳糊电极的葡萄糖传感器研究

Amperometric Glucose Sensor Based on Ferrocene-Nafion Chemically Modified Screen-printed Carbon Paste Electrode

以基于丝网印刷技术制作的厚膜碳糊电极为基底电机，用二茂铁为介体、Nafion修饰厚膜碳糊电极制备了葡萄糖传感器．Nafion涂于电极表面上形成的膜具有强的附着力，防止了二茂铁及酶的流失，电极稳定性提高．并且由于低的工作电位（＋025vsSCE）和Nafion膜的阳离子交换作用，基本上消除了电活性物质（抗坏血酸、尿酸）的干扰，具有防污能力．该酶传感器的检测上限可达18mmol／L，响应时间小于60s.

Amperometric mediated glucose biosensor has been developed by immobilizing GOD on Nafion-Fc chemically modified screen-printed carbon electrode, It is further coated by Nafion film. Ferrocene as a redox mediator can catalyze the oxidation of enzymatic generated H2O2 at a favorable lower working potential (ca 0. 25V vs SCE). The modified Nafion film can be used to avoid the leakage of ferrocene and GOD fronl biosensor. The interference ofelectroactive species, such as ascorbic acid and uric acid,can be also decreased. The upper detection limit of designed glucose sensor is 18. 0mmol/L, and the response time is less than 60s. This enzyme electrode exhibits a good stability and reproducibility,