FTIR对TFAR19基因促红白血病MEL细胞凋亡作用的研究

FTIR Spectroscopy Studies on the Apoptosis-promoting Effect of TFAR19 on the Erythroleukemia Cell Line MEL

采用 FTIR方法 ,研究了 TFAR19基因转染前后 ,小鼠红白血病 MEL 细胞内重要组分的变化。经脂质体介导基因转染 ,获得了稳定携带 TFAR19基因的 MEL- TF19细胞 ,RT- PCR检测表明 ,该基因在 m RNA水平有表达 ;撤除血清诱导凋亡 ,在此过程中进行了 FTIR检测 ,结果显示 :转染 TFAR19基因后 ,细胞内蛋白质相对核酸的含量增高 ,细胞转录活性增强 ,细胞内磷脂的相对含量降低。所有这些变化都反映了 TFAR19基因的促凋亡作用 。

The changes in the cellular main components of the mouse erythroleukemia cell line MEL for TFAR19 gene transfection were studied by the technology of Fourier transform infrared spectroscopy(FTIR). Using the method of gene transfection with liposome, we obtained MEL-TF19 cell line, which stably carries TFAR19, a novel apoptosis-related gene. The expression of the gene on mRNA level was confirmed by RT-PCR. Then, FTIR spectra of the cells were measured in the course of apoptosis induced by serum deprivation. Our results indicated that after being transfected with TFAR19 gene, MEL-TF19 cells exhibited relatively higher protein content, higher transcriptional activity, and relatively lower phospholipid content as compared with those exhibited by MEL cells. All the above changes reflect the apoptosis-promoting effect of TFAR19 gene, and maybe account for the cellular rheological changes after TFAR19 gene transfection, which were discovered in our previous study.