人肝刺激因子的提取及其保肝作用的初步观察

Isolation of human hepatic stimulator substance and the preliminary study of its protective action(Abstract)

采用水囊引产眙儿(胎龄4～6个月),提取肝刺激因子(hHSS),用~3H—胸腺嘧啶核苷测其活性,观察到 hHSS 可使34%肝切除的大鼠对~3H—胸腺嘧啶核苷掺入再生肝 DNA 率明显增加。将 hHSS 预先注入半乳糖胺损伤的小鼠体内,结果观察到:hHSS 极明显地降低半乳糖胺中毒的死亡率(P<0.01),明显地抑制半乳糖胺所致的血清 GPT 和 GOT 的高水平。电镜检查表明,hHSS 可保护肝细胞的超微结构(线拉体、内质网等)免受半乳糖胺的损伤。这些结果证明,从人胎肝中可提取 hHSS,而 hHSS 具有保护肝脏免受肝毒剂—半乳糖胺的毒害作用。

The human hepatic stimulator substance(hHSS)had been isolated and partial purified from human fetal liver(4-6 month age),and biological activity of hHSS was demonstrated by means of^3H-thymidine incorpo- ration into hepatocyte DNA when hHSS injected intraperitoneally into 34% hepatectomy rats.We further observed that hHSS could decrease the mortality induced by D-galactosamine poisoning mice,and suppress the high level of sGPT and sGOT evoked by D-galactosamine intoxica- tion.The electromicroscope examination show that hHSS lessen the structural damage of liver induced by D-galactosamine.The above- mentioned results suggest that hHSS may be isolated from human fetal liver,and it has dramatic protective effect on liver against D-galacto- samine injure.