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Laser-induced tobacco protoplast fusion

Laser-induced tobacco protoplast fusion
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摘要 Laser tweezers can manipulate small particles, such as cells and organdies. When coupling them with laser microbeam selective fusion of two tobacco protoplasts containing some chloroplast was achieved. Physical and biological variables that affect laser trapping and laser-induced fusion were also discussed. The results show that the effect of chloroplast content and distribution on the yield of cell fusion is remarkable. Laser tweezers can manipulate small particles, such as cells and organelles. When coupling them with laser microbeam selective fusion of two tobacco protoplasts containing some chloroplast was achieved. Physical and biological variables that affect laser trapping and laser-induced fusion were also discussed. The results show that the effect of chloroplast content and distribution on the yield of cell fusion is remarkable.
出处 《Science China(Life Sciences)》 SCIE CAS 1999年第2期122-127,共6页 中国科学(生命科学英文版)
基金 Project supported by the National Natural Science Foundation of China (Grant No. 39470205) State Science and Technology Commision, and Science and Technolgy Commision of Anhui Province
关键词 LASER TWEEZERS LASER MICROBEAM TOBACCO PROTOPLAST CHLOROPLAST cell fusion. laser tweezers, laser microbeam, tobacco protoplast, chloroplast, cell fusion.
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