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Expression of. Arabidopsis tryptophan biosynthetic pathway genes: effect of the 5' coding region of phosphoribosylanthranilate isomerase gene 被引量:1

Expression of. Arabidopsis tryptophan biosynthetic pathway genes: effect of the 5' coding region of phosphoribosylanthranilate isomerase gene
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摘要 There are three non-allelic isogenes encoding phosphoribosylanthranilate isomerase (PAI) in Arabidopsis thaliana. The expression plasmids were constructed by fusion of the GUS reporter gene to the three PAI promoters with or without the 5' region encoding PAI N-terminal polypeptides and transferred into Arabidopsis plants by Agrobacterium tumefaciens. Analysis of GUS activity revealed that the PAI 5' coding region was necessary for high expression of GUS activity. GUS activity in transgenic plants transformed with the expression plasmids containing the 5' coding region of PAH or PAD was 60-100-fold higher than that without the corresponding 5' region. However, the effect of 5' coding region of PAI2 gene on the GUS activity was very small (only about 1 time difference) . The GUS histochemical staining showed a similar result as revealed by GUS activity assay. It was expressed in the mesophyll cells and guard cells, but not in the epidermic cells, indicating that the N-terminal polypeptides encoded by the 5' region of PAI genes have the function of PTP. There are three non-allelic isogenes encoding phosphoribosylanthranilate isomerase (PAI) in Arabidopsis thaliana. The expression plasmids were constructed by fusion of the GUS reporter gene to the three PAI promoters with or without the 5’ region encoding PAI N-terminal polypeptides and transferred into Arabidopsis plants by Agrobacterium tumefaciens. Analysis of GUS activity revealed that the PAI 5’ coding region was necessary for high expression of GUS activity. GUS activity in transgenic plants transformed with the expression plasmids containing the 5’ coding region of PAI1 or PAI3 was 60—100-fold higher than that without the corresponding 5’ region. However, the effect of 5’ coding region of PAI2 gene on the GUS activity was very small (only about 1 time difference). The GUS histochemical staining showed a similar result as revealed by GUS activity assay. It was expressed in the mesophyll cells and guard cells, but not in the epidermic cells, indicating that the N-terminal polypeptides encoded by
出处 《Science China(Life Sciences)》 SCIE CAS 1999年第3期274-280,337-338,共9页 中国科学(生命科学英文版)
基金 Project supported by the National Distinguished Young Scholar Grant
关键词 phosphoribosylanthranilate ISOMERASE (PAI) TRYPTOPHAN biosynthetic pathway GENE EXPRESSION and regulation PLASTID targeting peptide (PTP) ARABIDOPSIS thaliana. phosphoribosylanthranilate isomerase (PAI), tryptophan biosynthetic pathway, gene expression and regulation, plastid targeting peptide (PTP), Arabidopsis thaliana.
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