摘要
By means of eDNA-RDA)A method, some cd)YA fragment- were found to have high levels oi expression during deprivation of GM-CSF ( granulocyte macrophage-rolony stimulating (actor) in a human myeloid cell line, TF-I cells. One of these fragments was identified as a novel gene. To gel the full length of eDNA, rapid amplification of cDYA ends (RACE) and expressed sequence tags ( EST) overlapting fragments assembling strategies were used. The novel gene was named TRAF15 (TF-1 cell apoptosis related gene-15) , which consist of I 218 nucleotides and eneodes 212 ammo acids. The putative protein product oi TFAR15 is parlially homologous to C. elegans protein C14A4. 11 . TFAR15 mRNA is expressed in fetal liver, kidney, spleen and lung. and also in some human myeloid cell lines. Both of the TFAR15 mRNA and protein in wen: highly expressed in TF-1 cells after GM-CSF withdrawal. In vitro analysis showed that the tecombinant TFAR15 protem coold inhibit the natural cell death of 293 cells, an embryonie kidney cell line.
By means of cDNA-RDA method. some cDNA fragments were found to have high levels of expression during deprivation of GM-CSF (granulocyte macrophage-colony stimulating factor) in a human myeloid cell line, TF-1 cells. One of these tragments was identified as a novel gene. To get the full length of cDNA, rapid amplification of cDNA ends (RACE) and expressed sequence tags (EST) overlapping fragments assembling strategies were used. The novel gene was named TRAF15 (TF-1 cell apoptosis related gene-15), which consists of 1218 nueleotides and encodes 212 amino acids. The putative protein protein product of TFAR15 is partially homologous to C. elegans protein C14A4. 11. TFAR15 mRNA is expressed in fetal liver, kidney, spleen and lung. and also in some human myeloid cell lines. Both of the TFAR15 mRNA and protein were highly expressed in TF-(?) cells after GM-CSF withdrawal. In vitro analysis showed that the recombinant TFAR15 protein co(?)ld inhibit the natural cell death of 293 cells, an embryonic kidney
