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Effect of DNA methylation on protein-DNA interaction of HL-60 cells

Effect of DNA methylation on protein-DNA interaction of HL-60 cells
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摘要 HL-60 cells have been induced with differentiation index 16% by S-adenosyl-L-methionine (SAM) as in-ducer in the presence of optimum concentration of 10 μmol/L. The methylation level of genome DNA determined by HPLC is increased during cell differentiation. When restriction endonuclease Hae Ⅲ, Sma Ⅰ, Sal Ⅰ, Xho Ⅰ and Hind III which are sensitive to 5-methylcytosirte were used to cleave the genome DNA, a resistance effect was found. The interaction between DNA and DNA binding proteins is changed by using gel retarding test. HL-60 cells have been induced with differentiation index 16 % by S-adenosyl-L-rnethionine (SAM) as inducer in the presence of optimum conceptration of 10 μmol/L. The methylation level of genorne DNA determined by HPLC is increased during cell differentiation. When restriction endonuclease Hae Ⅲ, Sma I, Sal I, XhoI and Hind Ⅲ which are sensitive to 5-methylcytosine were used to cleave the genorne DNA, a resistance effect was found. The interaction between DNA and DNA binding proteins is changed by using gel retarding test.
出处 《Science China(Life Sciences)》 SCIE CAS 1999年第5期501-505,共5页 中国科学(生命科学英文版)
关键词 S-ADENOSYL-L-METHIONINE (SAM) HL-60 cell DNA METHYLATION PROTEIN-DNA interaction. S-Adenosyl-L-methionine (SAM), HL-60 cell, DNA methylation, protein-DNA interaction.
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