肌苷对脑缺血再灌流损伤神经功能恢复和NSE表达的影响

Effects of inosine on neurological function recovery and expression of neuron-specific enolase after cerebral ischemia reperfusion

目的 观察大鼠脑缺血再灌流损伤脑组织神经元特异性烯醇化酶 (NSE)的变化规律及其意义 ;探讨肌苷对缺血性脑损伤的保护机制。方法 用成年 SD大鼠建立大脑中动脉缺血 (MCAO)再灌流模型 ,腹腔注射肌苷注射液 ,应用神经功能等级评分观察脑缺血再灌流后行为功能的恢复 ,免疫组化法检测脑缺血再灌流各时间点脑组织中 NSE的动态变化。结果 对照组脑缺血再灌流后 3d、7d、1 4 d功能恢复、等级评分减低 ;缺血侧 NSE的免疫阳性反应于脑缺血再灌流后 1 2 h明显增强并达高峰 ,随时间推移逐渐下降 ,至 1 4 d降至假手术组水平。治疗后神经功能评分在 7～1 4 d明显低于对照组 ,同时脑组织中 NSE蛋白表达水平较对照组显著升高。两组中皮层区 NSE的免疫阳性反应均高于纹状体区。结论 肌苷对缺血性脑损伤的功能恢复有一定的促进作用 ,其机制可能与增加脑组织中

Objective To study the expression and significance of neuron specific enolase (NSE) in the cerebral tissue after reperfusion of focal cerebral ischemia, and to elucidate the protective mechanism of inosine in hypoxic ischemic brain damage Methods The models of reperfusion of focal cerebral ischemia of SD rats were established by intraluminal middle cerebral artery occlusion (MCAO) with a nylon monofilament suture Inosine was injected intraperilonoally twice before and 12h after reperfution Neurological grade evaluation was applied to examine the behavioral recovery and immunohistochemical technique was used to detecte the dynamic changes of NSE in cerebral tissue on the different time of cerebral ischemia reperfusion Results There was improvement in the score of neurologic grade in inosine treatment group on the 7th and 14th after reperfusion compared with the controlls The expression of NSE in ischemia part peaked at 12h, and then decreased gradually, and to the fame operation group level on the 16th after reperfusion In treatment group, the neurologic grade decreased while the expression of NSE protein increased remarkablely in cerebral tissue compared with controlled group Conclusions Inosine could protect cerebral tissue against hypoxic ischemic brain damage, whose role might be reflected by increasing the expression of NSE