流行性出血热患者肾肝穿刺组织原位分子杂交

In Situ hybridization cf opidemic hemorrhagic fever kidney and liver tissues

目的:探讨流行性出血热病毒(EHFV)在患者体内感染的靶器官。方法:应用原位分子杂交技术检测了流行性出血热(EHF)10例肾穿组织和3例肝穿组织中病毒RNA的定位和分布。结果:肾、肝组织上皮细胞和血管内皮细咆及肝窦内皮细胞均表达阳性,但肾近端小管上皮细胞较远端小管上皮细胞、血管内皮细胞、肝细胞和肝窦内皮细胞表达要强。多数病人肾近端小管上皮细咆出现病毒包涵体。结论:肾近端小管是EHF病毒直接攻击的靶细胞,损伤最严重。所以,对EHF病人保护肾功能是治疗的关键。

In order to study the target cells of epidemic hemorrhagic fever rirus(EHFV)in the patients , localix-ation and distribution of virus RNA was performed on ten kidneys and three liver tissues of EHF patients by in situ hybridization .The reustls show that epithelial cells of carpillary and endothelial cells of hepatic sinus are positive expression at all , epithelial cells of kidney proximal tubule are the strongest positive expression. The uiral inclusion bodies are seen in most kidney proximal tubules of EHF patients. The results suggest that proximal tubule epithelial cell is susceptible target organ of EHFV and is injured easy byit Therefore, to protecting the function of kidney in EHF patient is the most important treatment.