表皮生长因子对肺表面活性物质合成的调控

Modulation of Epidermal Growth Factor on Pulmonary Surfactant Synthesis

采用无血清成年大鼠肺组织培养，液闪测3H-胆碱掺入PC量，现察hEGF对成年大鼠PS合成的影响。结果显示：①hEGF促进肺组织3H-胆碱掺入量，量-效关系显著。②10－9mol/LhEGF作用8h后，可显著增加3H-胆碱掺入量P＜0．01），16h达高峰。③PKC抑制剂10－5mol/LH7可显著降低10－9mol/LhEGF组3H-胆碱掺入量（P＜0．01）。④hEGF对肺组织LDH释放量无显著影响，提示本实验各处理因素对肺组织无细胞毒性。结果表明EGF可促进成年大鼠PS合成，PKC参与介导EGF促PS合成效应。

The effect of hUman epidennal growth factor on PS synthesis was studied in cultured ratlung explants without serum, and amount of 3H-choline incorporation into PC wasdetermined. The results showed that: rohEGF increased 3H-chollne inocrporation inadedependent rnarmer. ②10-9mol/L hEGF increased 3H-choline incomption into PC after sh(P < 0. 05). ③H7,a PKC ichbitor decreased 3H-choline incorporation induced by(P < 0. 01). ④the release of LDH from lung tissues could not be changed by hEGF. Theresults showed that EGF could enhance the adult PS synthesis mediated by PKC.