含SV_(40)串联启动子红细胞生成素逆转录病毒表达载体的构建

Cloning of Human Erythropoietin Genome with SV_(40) Series Promoter in A Retroviral Vector

本文构建了含SV40串联启动子的红细胞生成素（EPO）逆转录病毒表达载体。因逆转录病毒载体pN2A需用外源启动子才能表达目的的基因，故把SV40串联启动子及包括除信号肽中第2、3、4位氨基酸外的所有编码区及两个内含子序列的EPO基因片段一起克隆到pN2A载体neo基因下游的多聚接头上的单一酶切位点BglⅡ上，构建成重组质粒pN2ASV40EPO。

In order to obtain an erythropoieton(EPO) expressing vector,EPO genom with SV40 seriespromter was cloned into a retroviral vector pN2A. As foreign proter is needed for pN2A to express the target gene, the SV40 series promoter, all the driing region except the secod, third andfourth arnino acids in the signal Peptide and two introns of EPO genome were cloned togther intothe Bgl Ⅱ site of the Polylinker of pN2As neo gene downstream, thus a recominant plasmidpN2ASV40EPO was constructed.