鼻咽癌患者淋巴细胞染色体断裂热点与脆性部位、癌基因位点相关性的初步探讨

The Study on the Relationship of the Induced Chromosome Breakpoints in Peripheral Blood Lymphocytes of Nasopharyngeal Carcinoma with the Fragile Site and Oncogenes Loci in Human Chromosomes

为了探索人类染色体脆性部位与肿瘤发生的关系．运用染色体原位G显带方法．观察和分析21例鼻咽癌患者和30例健康对照者淋巴细胞在常规培养条件下，自发和诱发染色体断裂热点与脆性部位、及其与癌基因位点的相关性。实验结果表明，二亚硝基哌嗪诱发鼻咽癌患者淋巴细胞染色体断裂热点与脆性部位和癌基因位点相关（P＜0．05），而EB病毒诱发的热点与脆性部位相关（P＜0．05），与癌基因位点不相关（P＞0．05）。提示环境致癌物可能增加FS表达，脆性部位和癌基因位点在肿瘤发生中可能作为环境致癌物作用的靶子发挥作用。诱发的正常人与鼻咽癌患者染色体断裂热点FS和ONC位点相关性的差异可能反映了二者对环境致癌剂敏感性的不同。

This investgation sought to address if there was any association between tumer genetic susceptibility and human chromosome fragile sites (FS). Using the method of G-banding in situ,theassociation among the spontaneous and induced chromosome breakpoints of peripheral blood lymphocytes (BP) of 21 nasopharyngeal carcinoma(NPC) patients and 30 health person by usual culture of blood and FS and oncogenes (ONC) loci. The results showed there was association between the BP induced by dinitrosopiperazin(DNP) in NPC patients group and FS(P < 0. 05).And there was association between this BP and ONC loci (P < 0.05). There was associationbetween the BP induced by Epstein-Barr virus (EBV) in NPC patients and FS (P < 0. 05).And there was no association between this BP and ONC loci (P > 0. 05),This investigation suggests that there may be associatiom between tumor genetic susceptibility and FS.