支气管肺泡灌洗液聚合酶链式反应与腺苷脱氨酶检测肺结核的临床意义

Clinical Significance of PCR of BALF and ADA for the Detection of Pulmonary Tuberculosis

目的：通过观察支气管肺泡灌洗液（BALF）的聚合酶链式反应（PCK）与腺苷脱氨酶（ADA）检测，探讨诊断肺结核的临床意义．方法：同时分别对肺结核和支气管内结核（结核组）30例、支气管炎和支气管扩张（支炎组）42例、支气管肺癌（肺癌组）79例、食道癌和胃癌等（肺外肿瘤组）21例进行检测．结果：四组BALF的TB—DNA—PCR检测阳性率分别为76％、28．6％、31％、9．1％，经X2检验结核组对其它三组差异有显著意义（P＜0．001），BALF的ADA检测分别为11．52±8．26、12．47±8．64、10．82±11．34、10．39±11．11，经t检验无显著差异性（P＜0．05）．结论：提示BALF的TB—DNA—PCR检测对诊断结核病具有高敏感性及特异性．

To investigate the clinical significanceof the disgnosis of pulmonary' tuberculosis by observing polymerase chain reaCtion (PCR) Of bronchopulmonary alveoli lavage, fluid (BALF) and thedetection of adencoine deaminase (ADA). Method30 cases Of pulmonary tuberculosis and endobronchial tuberculosis, 42 cases of bronchitis andbronchiectasis, 79 cases of broncho-pulmonary carcinoma and 21 cases of carcinoma of esophagus andcarcinoma of stomach were respectively detected.Results The positive rate of detection of TB-DNA-PCR of BALF in the four groups were 76%.28.6%.31%.91.% respectively, which had significantdifference between tuberculosis and the other threegroups (P<0.01).The deteCtion of ADA of BALFin the four groups were 11. 52±8.26.12.47±8. 64.10. 82±11.34 and 10.39±11.11 respctively,whichshowed no significant difference among them(P>0.05). Conclusion It suggests the detection of TB DNA - PCR of BALF has hypersensitivity andspecificty for the diagnosis of tubercutois.