摘要
以翠菊(Callistephus chinensis)叶片为外植体,在 MS+6—BA0.5mg/L+IAA2.0mg/L的培养基上进行培养、结果发现,每叶片平均再生芽数4～6个,再生率达80%～85%.外源激素和光照是影响芽分化的关键因素.
Using excised leaf disk of Callistephus chinensis as the explant,they were cultured on MS+6—BA0.5mg/L+IAA2.0mg/L.The experimental results indicated that regener- ation of leaf disk reached 80%—85% with an average of 4—6 shoots per leaf disk. Exgenous hormone and lux were important.
出处
《沈阳师范大学学报（自然科学版）》
CAS
1998年第4期55-57,共3页
Journal of Shenyang Normal University:Natural Science Edition
关键词
翠菊
组织培养
植株再生
Callistephus chinensis
tissue culture
plant regeneration
