精氨酸加压素对血管平滑肌细胞脂质过氧化的影响及辅酶Q10的调节作用

Effect of Arginine Vasopressin on Lipid Peroxide of Vascular Smooth Muscle Cells and Its Regulation by Coenzyme Q10

目的：探讨精氨酸加压素（ＡＶＰ）与高血压发病和血管平滑肌细胞（ＶＳＭＣ）脂质过氧化的关系以及ＣｏＱ１０的干预作用。方法：培养大鼠主动脉ＶＳＭＣ，测定其脂质过氧化物（ＬＰＯ）含量，观察ＡＶＰ对ＶＳＭＣ脂质过氧化的影响和ＣｏＱ１０对此作用的调节。结果：①ＡＶＰ有明显的促ＶＳＭＣ脂质过氧化作用，且时间愈长，作用愈显著；②ＣｏＱ１０对ＡＶＰ的促ＬＰＯ生成有明显的抑制作用，并随浓度增高作用增强。结论：ＡＶＰ参与高血压发病可能与其促ＶＳＭＣ脂质过氧化作用有关；ＣｏＱ１０具有抑制ＡＶＰ促ＶＳＭＣ脂质过氧化作用。

Purpose:To study the relationships between:(1)arginine vasopressin(AVP) and the pathogenesis of hypertension;(2) AVP and lipid peroxide(LPO), and to explore the regulative action of coenzyme Q10(CoQ10) on them. Methods: By using the cultured aortic vascular smooth muscle cells(VSMC) of rats, the content of LPO and its changes in VSMC induced by AVP were detected, as well as the regulative action of CoQ10 on them.Results:(1) AVP significantly enhanced the production of LPO in VSMC, the longer the time,the more apparent the effect; (2) CoQ10 significantly inhibited the effect of LPO production in VSMC induced by AVP, the higher the CoQ10 concentration, the more apparent the effect.Conclusion: The effect of AVP on pathogenesis of hypertension may be related with its enhancement of production of LPO in VSMC. CoQ10, which could inhibit the effect of AVP, may has an important significance in prevention and treatment of hypertension.